Dancing With The Stars won outstanding reality TV programme for featuring contestant Chaz Bono, the transgender child of singers Cher and Sonny Bono.
Lady Gaga won outstanding music artist for her album Born This Way.
Glaad promotes positive images of lesbian, gay, bisexual and transgender (LGBT) people in the media.
The producers of new TV musical drama series Smash picked up the Vito Russo award at the ceremony.
Winners Neil Meron and Craig Zadan are also behind movies including Oscar-winning musical Chicago and Hairspray.
The prize is named after the late gay rights activist and presented to an openly LGBT media professional who has made a significant contribution to promoting equality.
Zadan said being gay was "part of who I am, so it impacts the work that we do".
Meron said he and Zadan had spent years trying to "fight, persuade and manipulate" the entertainment industry to include lesbian, gay, bisexual and transgender characters and stories.
Craig Zadan (L) and Neil Meron Zadan and Meron's first big TV success was Gypsy starring Bette Midler in 1994.
They also recounted how they had received death threats following their controversial 2003 mini-series The Reagans, which argued that former US President Ronald Reagan failed to recognise the Aids crisis.
Hollywood producer Harvey Weinstein presented openly gay high school pupil Katy Butler with a special award for spearheading a campign to persuade US film censors to lower its rating for documentary Bully.
HBO's Cinema Verite, meanwhile, picked up the best TV movie or mini-series award.
She tweeted: 'People ask me why I wear veils. I reply, I'm mourning. Mourning what? Well I figure something shy must be going on somewhere.'
Last November, Gaga, 25, launched her own charity for youth empowerment, the Born This Way Foundation.
The eccentric star set up the not-for-profit group - partnered with groups including the John D. and Catherine T. MacArthur Foundation and the Berkman Center for Internet & Society at Harvard University - to help people worldwide develop a new standard of care towards one another.
She said: 'Together we hope to establish a standard of bravery and kindness, as well as a community worldwide that protects and nurtures others in the face of bullying and abandonment.'
Gaga has fronted many campaigns for charity and ethical issues, previously speaking to gay rights campaigners about the repealing of the don't ask, don't tell policy in the US - which didn't allow openly homosexual officers to serve in the armed forces - before its repealing earlier this year.
The 'You and I' singer is currently preparing for the launch of her 'Born This Way Ball' world tour, which starts at the end of next month in Seoul, South Korea.
It starred Tim Robbins, Diane Lane and James Gandolfini and was based on the 1973 PBS American Family documentary that featured the first openly gay person on television.
Best talk show episode went to Oprah Winfrey's programme for the episode Coming Out on the Oprah Show: 25 Years of Unforgettable Guests.
Playwright Tony Kushner won the theatre award for his play, The Intelligent Homosexual's Guide to Capitalism and Socialism with a Key to the Scriptures.
